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Immunocytochemistry For intestinal organoids cultured on coverslips were washed with ice-cold PBS and fixed in 4% paraformaldehyde
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First, pathway complexity raises on- and off-target risks when modulating ASC, caspase-1, GSDMD, IL-1, NF-B, P2X7R, IKK, FTH1, ACSL4, miR-204, or GPX4, and microglia-specific target engagement is difficult to confirm
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