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These results are in accordance with our previous study showing that smoking negatively alters the sperm standard parameters, DNA stability of sperm, and the ratio of protamine mRNA as well as downregulates the expression of H2BFWT, PRM1, and PRM2 36

WHO established MPOWER and it means: M: Monitor tobacco use and prevention policies P: Protect people from tobacco smoke O: Offer help to quit smoking W: Warn about the dangers of tobacco E: Enforce bans on tobacco advertising, promotion, and sponsorship R: Raise taxes on tobacco Support tobacco farmers to switch to alternative, sustainable livelihoods, in line with Art 17 and 18 of the WHO Framework Convention on Tobacco Control (FCTC)

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Flow cytometric detection of the mitochondrial BCL2 protein in normal and neoplastic human lymphoid cells