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The majority of cell surface receptors are glycosylated/sialylated, and hyposialylation of muscle glycoproteins is thought to be responsible for muscle deterioration in mouse models of GNE myopathy 17,18,19,20

Differentiation and transfection of bovine intramuscular preadipocytes Bovine intramuscular preadipocytes were cultured in 6-well plates in a 5% CO incubator (Thermo Fisher Scientific) at 37 C until they reached 80% confluence