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But one of the important things that you'll find interesting that I published on back in 2014 was that I'd gone through this big published database where someone had published all these genes they found VDREs in

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After filtration with 200 mesh screen and centrifugation at 1500 rpm, the precipitates were inoculated in Dulbeccos modified Eagles medium (DMEM) complete medium containing 1% penicillinstreptomycin and 10% FBS and cultured at 37C and 5% CO 2 saturation humidity for 1.5 h, and then, the nonadherent cells were collected

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